Kaempferide improves glycolipid metabolism disorder by activating PPAR? in high-fat-diet-fed mice

Kaempferide (Ka, 3,5,7-trihydroxy-4?-methoxyflavone), an active ingredient of Tagetes erecta L., has been demonstrated to possess many pharmacological effects, including antioxidant, anti-inflammation, anticancer and antihypertension in previous study. However, there is no evidence Ka on metabolic disorder former studies. This study investigated the effects glycolipid metabolism explored underlying mechanisms action vivo vitro. The mouse model was induced by high-fat diet (HFD). were evaluated bodyweight, lipid glucose metabolism. Hypolipidemic effect examined blood sample analysis. hypoglycemic detected several indicators, like glucose, serum insulin, HOMA index intraperitoneal tolerance tests (IPGTT). signaling pathways (PPAR?/LXR?/ABCA1) (PPAR?/PI3K/AKT) using Real-Time PCR Western blot. primary culture hepatocyte prepared confirm target co-culturing with PPAR? agonist or inhibitor. HFD mice developed obesity, hyperlipidemia, hyperglycemia insulin resistance. Administration at a dose 10 mg/kg.BW for 16 weeks effectively attenuated these changes. Further studies revealed hypolipidemic depended activation PPAR?/LXR?/ABCA1 PPAR?/PI3K/AKT pathways, respectively. test, co-cultured agonists inhibitors, further confirmed above pathway key protein. These results suggested that played important role improving disorder. favorable causally associated anti-obesity. might have do its downstream pathway. Our helped understand actions Ka, effective treatment diabetes, nonalcoholic hepatitis other diseases.